Use of a variable electrical pulsing sequence in rabbit oocyte activation
نویسندگان
چکیده
منابع مشابه
Use of a variable electrical pulsing sequence in rabbit oocyte activation.
Variability in oocyte activation sensitivity to electrical stimuli was shown in two types of oocytes (i.e., oocytes with a whole first polar body: w-PB 1 and those with a fragmented PB 1: f-PB 1), of a similar post-ovulatory age. In order to initiate the normal activation display (i.e., extrusion of the second polar body), the w-PB 1 oocytes required, on average, 3.6 +/- 0.2 pulses and the f-PB...
متن کاملInfluence of sequence duration and number of electrical pulses upon rabbit oocyte activation and parthenogenetic in vitro development.
Electroactivation of in vivo mature young rabbit oocytes was investigated here. The effects of four or eight electrical pulse treatment over 90, 150 or 270 min upon oocyte activation frequency and type, and even upon their subsequent in vitro development, were studied. The lowest activation frequency was observed after applying four-pulses over 90 min (54%). However, extending four-pulse treatm...
متن کاملP-97: Optimized Combined Electrical-Chemical Parthenogenetic Activation for Zona Free Sheep Oocyte
Background: Artificial oocyte activation (AOA) has important implications in assisted reproductive technique (ART) with particular importance in somatic cell nuclear transfer (SCNT). Parthenogenesis activation using zona- free oocytes offers an alternative model that could be applied to develop protocols for the activation of reconstructed embryos for cloning. Materials and Methods: To establis...
متن کاملSuccessful fertilization and pregnancy following ICSI and electrical oocyte activation.
In a total of 1048 intracytoplasmic sperm injection (ICSI) cycles, motile spermatozoa from four out of 424 patients (0.9%) failed to fertilize oocytes, despite an apparently successful ICSI procedure. No activation was observed in these injected oocytes. The spermatozoa from three of the four patients were injected into unfertilized mouse oocytes by ICSI (mouse test) to evaluate their oocyte ac...
متن کاملInverting polar domains via electrical pulsing in metallic germanium telluride
Germanium telluride (GeTe) is both polar and metallic, an unusual combination of properties in any material system. The large concentration of free-carriers in GeTe precludes the coupling of external electric field with internal polarization, rendering it ineffective for conventional ferroelectric applications and polarization switching. Here we investigate alternate ways of coupling the polar ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction Nutrition Development
سال: 2000
ISSN: 0926-5287,1297-9708
DOI: 10.1051/rnd:2000130